Use: Frozen & paraffin sections & IHC. Newcomer Supply Hematoxylin Stain, Gill, is a ready to use progressive nuclear stain available in three strengths for cytology and histology applications. Due to the progressive staining nature of Gill hematoxylin, over-staining is less likely and a differentiation step is not required. Gill hematoxylin does not require filtering but if using any Gill hematoxylin for cytology specimen staining, filtering before each use is highly suggested to prevent solution contamination. Gill II, noted as “double strength”, used as a counterstain in immunohistochemistry (IHC) procedures, frozen section nuclear stain, routine hematoxylin and eosin (H&E) stain and more intense cytology staining. Shelf Life is 1 year from date of manufacture. Store at 15-30°C.